The effect of exercise training and resveratrol supplementation with low fat diet on macrophage infiltration in adipose tissue of obese mice.

2016 한국운동생리학회 동계국제학술대회(샘플)

Hong Gil Dong

exephysio1@hanmail.net (교신저자 이메일 꼭 기재 요망)

OO University

Abstract

2012 한국운동생리학회 동계국제학술대회(샘플)

This study was to investigate the effect of exercise training and resveratrol supplement with a low-fat diet(LFD) on macrophage infiltration and inflammatory cytokine in adipose tissue of obese mice. All the mice(C57Bl/6) were divided into five groups; high-fat diet(HD), low-fat diet control(HLC), low-fat diet exercise(HLE), low-fat diet resveratrol(HLR), and low-fat diet exercise plus resveratrol(HLER). All the mice were fed a HFD(45% from fat of total calories) for 12 weeks  and then changed into LFD(10% from fat of total calories) for 7 weeks except HD group. Exercise mice ran on a treadmill at 12-20m/min for 50-60min/day for 7 weeks. Resveratrol supplement mice were orally gavaged with resveratrol(10㎎/㎏ of body weight) dissolved in 50% propylene glycol. Tumor necrosis factor(TNF)-α, interleukin(IL)-6, F4/80, monocyte chemoattractant protein(MCP)-1, inter-cellular adhesion molecule (ICAM)-1, vascular-cellular adhesion molecule(VCAM)-1, CD11c, CD163 and toll-like receptor(TLR)4 mRNA expressions were measured by RT-PCR. A comparison with HD mice, the body weight and epididymal fat mass were reduced in all treatment groups. F4/80, TNF-α, and IL-6 mRNA expression were decreased in HLR, HLE, and HLER mice compared to HD mice. CD11c mRNA expression was reduced by HLE, HLER mice compared to HD mice. In conclusion, exercise training with low fat diet has positive effect to attenuate the obese induced metabolic complication

Key words : obesity, macrophage, inflammation, exercise training, resveratrol